Calculating sperm concentrations
Calculating Sperm Count - www.nolixxxpic.com, Laboratory Continuing Education
The formula for calculating the sperm count, when 5 small squares within the large center square are counted is: Number of sperm counted x dilution.
This can be done by diluting the semen into a buffer containing a The dilution factor must be recorded to allow calculating the concentration.
Description:Capillary action will draw the fluid into the chamber. Be sure you count 5 squares on each side of the hemocytometer and then use the average for your calculations. In other words, to calculate the number of sperm per ml of original sample: Glossary Counting Cells with a Hemacytometer Hemacytometers were developed for counting blood cells, but can also be used to count spermatozoa. Thus, the volume over the central counting area is 0. You should be able to focus on the sperm but be careful not to break the coverslip. The tip of the pipette is placed in the V-shaped groove on the hemacytometer to load the sample into the chamber about 15 microliters.
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